个人基本信息
姓名：刘亮伟性别：男
职称：教授、博士生导师
邮编：450002
通信地址：河南省郑州市农业路63号, 河南农业大学生命科学学院
联系电话：0371-63555260
邮箱地址：liangwei@henau.edu.cn
个人简介
67.12生于河南省长葛市，87.9-91.7兰州大学生物学系学士毕业；02.2-05.7江南大学生物工程学院博士毕业；
05.7河南农业大学生命科学学院，05.12晋升副教授，12年晋升教授。
06年招收硕士研究生。
2012-2022“中国酶工程专业委员会委员”，国家自然科学基金评审专家、febs letters、global research journals of microbiology,生物物理学报、食品与生物技术学报等评审专家。主持国家自然科学基金、河南省重点科技攻关等项目。发表jbc、int j biol macromol , int. j. mol. sci. plos one、bmc biotechnology、process biochemistry、bbrc等sci论文。
教授课程
全校硕/博士研究生《生物信息学 bioinformatics》/《基因工程 genetic engineering》；生命学院本科生《基因工程》。09年《基因工程》“校级精品课程”，10年出版《基因工程原理与实验指导 principle of genetic engineering and experiment guide》，11/12年网上好评。
研究领域 (research direction) ：
从国家粮食安全问题入手，针对发酵工业、饲料业、农产品精深加工业中生物催化的关键酶类——木聚糖酶为模式分子, 开展酶分子“生物信息学研究(bioinformatics research)”和“理性改造 (rational engineering)”工作。进而开展连接酶、同源重组酶，针对基因重组、合成生物学、基因组编辑基本问题——dsb（double-stranded break）无缝（scarless）修复研究，达到“提高催化活性、提高转化效率、节约能源”目的。
科研项目 (projects)：
主持：国家自然科学基金面上项目（natural science foundation of china, 31771915）：“基于同源/非同源结构置换的木聚糖酶-葡聚糖酶结构元件功能解析”(functional analysis of structural elements based on homologous and non-homologous substitution between xylanase and glucanase, 2018-2021)主持：国家自然科学基金面上项目（natural science foundation of china, 31371831）：“基于结构分析的嗜热cbm 拆分-易位-组合融合对木聚糖酶稳定性-活性影响”(effect of thermophilic cbm disassembling-swapping-combinational fusing on xylanase stability-activity, 2014-2017)
主持：国家自然科学基金面上项目（natural science foundation of china, 3097212）：“基于ph相关氨基酸定位分析的双功能木聚糖酶耐酸性突变研究” (ph stability mutational research of bi-functional xylanase based on position analysis of related amino acids, 2010-2012)
主持：河南省重点科技攻关：“木聚糖酶热稳定性的全息酶工程定向改造”(2007-2009)
主持：河南省重点科技攻关：“饲料用葡聚糖酶-木聚糖酶双功能酶耐酸性分子改造” (2010-2012)
主要论著(papers)：
2022：
[1] xuegang li, jiacheng jin, wenxuan xu, mingdao wang, liangwei liu, abortive ligation intermediate blocks seamless repair of double-stranded breaks. int j biol macromol, 2022, 209:1498-1503.
[2] xuegang li, jiacheng jin, zhanyong guo, liangwei liu, evolution of plasmid-construction. int j biol macromol, 2022,209:1319-1326.
[3] 邵玉强, 金家铖, 李雪刚, 刘亮伟. 大量–高纯–高浓度dna的制备[j]. production of dnas in large-quantity, high-purification, and high-concentration 微生物前沿, 2022, 11(2): 67-74. https://doi.org/10.12677/amb.2022.112008
[4] 郑园霞, 张毅, 李雪刚, 刘亮伟. 荧光cy5dna 扩增及酶切产物分离-检测[j]. amplification of fluorescent cy5dna and separation-detection of digestion product, 微生物前沿, 2022, 11(4):241-250. doi: 10.12677/amb.2022.114030
2020：
[1] yaping liang, yu zhang, liangwei liu, intra-molecular homologous recombination of
scarless plasmid, int. j. mol. sci. 2020, 21, 1697; doi:10.3390/ijms21051697.
2018:
[1] 梁亚萍; 程晋生; 刘亮伟; 5’端与3’端间同源长度对质粒构建的影响, 河南科学, 2018,36(09):1383-1387. liang yaping，cheng jinsheng，liu liangwei, length effect of homologous 5’- and 3’-end on plasmid construction, henan science. 2018,36(09):1383-1387.
[2] yang, ang; cheng, jinsheng; liu, meng; shangguan, yunjie; liu, liangwei. sandwich fusion of cbm9_2 to enhance xylanase thermostability and activity. int j biol macromol, 2018,117: 586-591.
[3] 上官云杰; 梁亚萍; 杨昂; 程晋生; 黄亚威, 同源引物的非等量pcr, 河南科学, 2018, 36(03), 326-333. shangguan yunjie， liang yaping， yang ang， cheng jinsheng，huang yawei1， liu liangwei, homologous primer unequal pcr, henan science. 36(03), 326-333.
2017:
[1] 黄亚威，贺添艳，杨昂，上官云杰，徐文选，刘亮伟，双退火温度pcr扩增dna”，微生物学报，2017,57(8): 1262-1269. huang y, yang a, shangguan y, he t, xu w, liu l. amplification of dna with double annealing temperature pcr. acta microbiologica sincia. 2017, 57(8):1-8.
2016：
[1] wenxuan xu, yajuan liu, yanxin ye, meng liu, laichuang han, andong song, liangwei liu*, c-terminal carbohydrate-binding module 9_2 fused to the n-terminus of gh11 xylanase from aspergillus niger, biotechnology letters, 38(10), 1739-1745,
[2] 贺添艳 刘亚娟 徐文选 刘猛 刘亮伟，t4 dna连接酶性质及其平端连接功能，《河南科学》2016, 34,1058-1062. he tianyan, liu yajuan, xu wenxuan, liu meng, liu liangwei, property and blunt end ligation function of t4 dna ligase, henan science. 34, 1058-1062.
[3] 刘猛 刘亚娟 徐文选 贺添艳 刘亮伟，长片断引物反向pcr方法构建重复序列的重组质粒，《河南科学》2016,501-505. m liu, y liu, w xu, t he, l liu. construction of recombinant plasmid containing repeated sequence by megaprimer reverse pcr henan science. 34, 501-505 (2016).
[4] 刘亚娟 刘猛 徐文选 贺添艳 刘亮伟，peg影响pet20b质粒转化率，《淮阴工学院学报》2016, 25(3):30-33. liu yajuan，liu meng，xu wenxuan，he tianyan，liu liangwei, transformation efficiency of pet20b plasmid affected by peg, 2016, 25(3):30-33.
2015：
[1] 马闪闪 韩来闯 刘亚娟 刘猛 刘亮伟，cbm2蛋白的高密度自诱导：《中国农学通报》2015,18:140-145. ma shanshan, han laichuang, liu yajuan, liu meng, liu liangwei, high density self-induction of cbm2. chinese agricultural science bulletin, 2015,18:140-145.
[2] 韩来闯 马闪闪 刘亚娟 刘猛 刘亮伟，构建重组质粒的二步pcr方法，《河南科学》2015, 8:1321-1325. han l, ma s, liu y, liu m, liu l. construction of recombinant plasmid by two-step pcr. henan science, 2015, 33(8): 1321-1325.
2012：
[1] liangwei liu, linmin wang, zhang zhang, suya wang, and hongge chen. effect of codon message on xylanase thermal activity. j. biol. chem, 287(32): 27183-27188. 2012.
[2] liangwei liu, xiaofeng sun, pengfei yan, linmin wang, hongge chen. non-structured amino-acid impact on gh11 differs from gh10 xylanase. plos one, 7(9): e45762. 2012.
[3] liangwei liu, long chen, hui tian, li zhao. using signal peptide prediction with caution, a case study in aspergillus niger xylanase. process biochem, 47: 2527–2530, 2012.
[4] liangwei liu, linmin wang, zhang zhang, xiaodan guo, xiangqian li, hongge chen. domain-swapping of mesophilic xylanase with hyper-thermophilic glucanase. bmc biotechnology, 12:28, 2012,
[5] liangwei liu, lingyu zeng, suya wang, jie cheng, xiangqian li, andong song, kun wu, hongge chen. activity and thermostability increase of xylanase following transplantation with modules sub-divided from hyper-thermophilic cbm9_1-2. process biochem, 47:853-857, 2012.
2011：
[1] liangwei liu, guoqiang zhang, zhang zhang, suya wang, and hongge chen. terminal amino-acids disturb xylanase thermostability and activity. j biol chem, 286:44710-44715, 2011.
[2] liangwei liu, jie cheng, hongge chen, xiangqian li, suya wang, andong song, mingdao wang, bao wang, jinwen shen. directed evolution of a mesophilic fungal xylanase by fusion of a thermophilic bacterial carbohydrate-binding module. process biochem; 46: 395-398, 2011
2010：
刘亮伟，程洁，陈红歌，木聚糖酶碳水化合物结合结构域研究进展，生物工程学报，2010, 26:290-296. liu l, cheng j, chen h. xylanase carbohydrate binding module: recent developments. chin j biotech, 2010, 26(3): 290–296.
2009：
[1] liangwei liu, bao wang, hongge chen, suya wang, mingdao wang, shimin zhang, andong song, jinwen shen, kun wu, xincheng jia. rational ph-engineering of the thermostable xylanase based on computational model. process biochem; 44:912-915. 2009,
[2]刘亮伟，杨海玉，胡瑜，李相前，f/10木聚糖酶研究进展，食品与生物技术学报，2009,28(6)727-732
2007:
刘亮伟，秦天苍,王宝,刘全军,刘新育,王明道,木聚糖酶的分子进化，食品与生物技术学报，2007,26(6):110-118.
2006-2004
[1] liangwei liu，hongping dong, suya wang, hongge chen, weilan shao. computational analysis of dipeptides correlated with the optimal temperature in g/11 xylanase. process biochem 2006;41:305–311.
[2] liangwei liu, minglei wang, weilan shao and weijiang li. a novel model to determine the dipeptides responsible for optimum temperature in f/10 xylanase. process biochem 2005;40:1389–1394.
[3] liangwei liu, hongge chen, xincheng jia. the influence of secondary structure content on thermostability of f/10 xylanase. j biotech, 136:s201, 2008
[4] liangwei liu. the eleventh china-japan-korea joint symposium on enzyme engineering november 5-8, 2010, chengdu, china,
[5] liangwei liu, xiangqian li, xun li and weilan shao. computational analysis of responsible dipeptides for optimum ph in g/11 xylanase. biochem biophys res commun 2004; 321: 391– 396.
[6] liangwei liu, jue zhang, bin chen and weilan shao. principle component analysis in f/10 and g/11 xylanase. biochem biophys res commun 2004;322:277–80.
2．副主编：《固态发酵原理》化学工业出版社，2008，
3．副主编：《酶制剂工艺学》化学工业出版社，普通高等教育“十一五”规划教材，2008
[1] xuegang li, jiacheng jin, wenxuan xu, mingdao wang, liangwei liu, abortive ligation intermediate blocks seamless repair of double-stranded breaks. int j biol macromol, 2022, 209:1498-1503.
[2] xuegang li, jiacheng jin, zhanyong guo, liangwei liu, evolution of plasmid-construction. int j biol macromol, 2022,209:1319-1326.
[3] 邵玉强, 金家铖, 李雪刚, 刘亮伟. 大量–高纯–高浓度dna的制备[j]. production of dnas in large-quantity, high-purification, and high-concentration 微生物前沿, 2022, 11(2): 67-74. https://doi.org/10.12677/amb.2022.112008
[4] 郑园霞, 张毅, 李雪刚, 刘亮伟. 荧光cy5dna 扩增及酶切产物分离-检测[j]. amplification of fluorescent cy5dna and separation-detection of digestion product, 微生物前沿, 2022, 11(4):241-250. doi: 10.12677/amb.2022.114030
2020：
[1] yaping liang, yu zhang, liangwei liu, intra-molecular homologous recombination of
scarless plasmid, int. j. mol. sci. 2020, 21, 1697; doi:10.3390/ijms21051697.
2018:
[1] 梁亚萍; 程晋生; 刘亮伟; 5’端与3’端间同源长度对质粒构建的影响, 河南科学, 2018,36(09):1383-1387. liang yaping，cheng jinsheng，liu liangwei, length effect of homologous 5’- and 3’-end on plasmid construction, henan science. 2018,36(09):1383-1387.
[2] yang, ang; cheng, jinsheng; liu, meng; shangguan, yunjie; liu, liangwei. sandwich fusion of cbm9_2 to enhance xylanase thermostability and activity. int j biol macromol, 2018,117: 586-591.
[3] 上官云杰; 梁亚萍; 杨昂; 程晋生; 黄亚威, 同源引物的非等量pcr, 河南科学, 2018, 36(03), 326-333. shangguan yunjie， liang yaping， yang ang， cheng jinsheng，huang yawei1， liu liangwei, homologous primer unequal pcr, henan science. 36(03), 326-333.
2017:
[1] 黄亚威，贺添艳，杨昂，上官云杰，徐文选，刘亮伟，双退火温度pcr扩增dna”，微生物学报，2017,57(8): 1262-1269. huang y, yang a, shangguan y, he t, xu w, liu l. amplification of dna with double annealing temperature pcr. acta microbiologica sincia. 2017, 57(8):1-8.
2016：
[1] wenxuan xu, yajuan liu, yanxin ye, meng liu, laichuang han, andong song, liangwei liu*, c-terminal carbohydrate-binding module 9_2 fused to the n-terminus of gh11 xylanase from aspergillus niger, biotechnology letters, 38(10), 1739-1745,
[2] 贺添艳 刘亚娟 徐文选 刘猛 刘亮伟，t4 dna连接酶性质及其平端连接功能，《河南科学》2016, 34,1058-1062. he tianyan, liu yajuan, xu wenxuan, liu meng, liu liangwei, property and blunt end ligation function of t4 dna ligase, henan science. 34, 1058-1062.
[3] 刘猛 刘亚娟 徐文选 贺添艳 刘亮伟，长片断引物反向pcr方法构建重复序列的重组质粒，《河南科学》2016,501-505. m liu, y liu, w xu, t he, l liu. construction of recombinant plasmid containing repeated sequence by megaprimer reverse pcr henan science. 34, 501-505 (2016).
[4] 刘亚娟 刘猛 徐文选 贺添艳 刘亮伟，peg影响pet20b质粒转化率，《淮阴工学院学报》2016, 25(3):30-33. liu yajuan，liu meng，xu wenxuan，he tianyan，liu liangwei, transformation efficiency of pet20b plasmid affected by peg, 2016, 25(3):30-33.
2015：
[1] 马闪闪 韩来闯 刘亚娟 刘猛 刘亮伟，cbm2蛋白的高密度自诱导：《中国农学通报》2015,18:140-145. ma shanshan, han laichuang, liu yajuan, liu meng, liu liangwei, high density self-induction of cbm2. chinese agricultural science bulletin, 2015,18:140-145.
[2] 韩来闯 马闪闪 刘亚娟 刘猛 刘亮伟，构建重组质粒的二步pcr方法，《河南科学》2015, 8:1321-1325. han l, ma s, liu y, liu m, liu l. construction of recombinant plasmid by two-step pcr. henan science, 2015, 33(8): 1321-1325.
2012：
[1] liangwei liu, linmin wang, zhang zhang, suya wang, and hongge chen. effect of codon message on xylanase thermal activity. j. biol. chem, 287(32): 27183-27188. 2012.
[2] liangwei liu, xiaofeng sun, pengfei yan, linmin wang, hongge chen. non-structured amino-acid impact on gh11 differs from gh10 xylanase. plos one, 7(9): e45762. 2012.
[3] liangwei liu, long chen, hui tian, li zhao. using signal peptide prediction with caution, a case study in aspergillus niger xylanase. process biochem, 47: 2527–2530, 2012.
[4] liangwei liu, linmin wang, zhang zhang, xiaodan guo, xiangqian li, hongge chen. domain-swapping of mesophilic xylanase with hyper-thermophilic glucanase. bmc biotechnology, 12:28, 2012,
[5] liangwei liu, lingyu zeng, suya wang, jie cheng, xiangqian li, andong song, kun wu, hongge chen. activity and thermostability increase of xylanase following transplantation with modules sub-divided from hyper-thermophilic cbm9_1-2. process biochem, 47:853-857, 2012.
2011：
[1] liangwei liu, guoqiang zhang, zhang zhang, suya wang, and hongge chen. terminal amino-acids disturb xylanase thermostability and activity. j biol chem, 286:44710-44715, 2011.
[2] liangwei liu, jie cheng, hongge chen, xiangqian li, suya wang, andong song, mingdao wang, bao wang, jinwen shen. directed evolution of a mesophilic fungal xylanase by fusion of a thermophilic bacterial carbohydrate-binding module. process biochem; 46: 395-398, 2011
2010：
刘亮伟，程洁，陈红歌，木聚糖酶碳水化合物结合结构域研究进展，生物工程学报，2010, 26:290-296. liu l, cheng j, chen h. xylanase carbohydrate binding module: recent developments. chin j biotech, 2010, 26(3): 290–296.
2009：
[1] liangwei liu, bao wang, hongge chen, suya wang, mingdao wang, shimin zhang, andong song, jinwen shen, kun wu, xincheng jia. rational ph-engineering of the thermostable xylanase based on computational model. process biochem; 44:912-915. 2009,
[2]刘亮伟，杨海玉，胡瑜，李相前，f/10木聚糖酶研究进展，食品与生物技术学报，2009,28(6)727-732
2007:
刘亮伟，秦天苍,王宝,刘全军,刘新育,王明道,木聚糖酶的分子进化，食品与生物技术学报，2007,26(6):110-118.
2006-2004
[1] liangwei liu，hongping dong, suya wang, hongge chen, weilan shao. computational analysis of dipeptides correlated with the optimal temperature in g/11 xylanase. process biochem 2006;41:305–311.
[2] liangwei liu, minglei wang, weilan shao and weijiang li. a novel model to determine the dipeptides responsible for optimum temperature in f/10 xylanase. process biochem 2005;40:1389–1394.
[3] liangwei liu, hongge chen, xincheng jia. the influence of secondary structure content on thermostability of f/10 xylanase. j biotech, 136:s201, 2008
[4] liangwei liu. the eleventh china-japan-korea joint symposium on enzyme engineering november 5-8, 2010, chengdu, china,
[5] liangwei liu, xiangqian li, xun li and weilan shao. computational analysis of responsible dipeptides for optimum ph in g/11 xylanase. biochem biophys res commun 2004; 321: 391– 396.
[6] liangwei liu, jue zhang, bin chen and weilan shao. principle component analysis in f/10 and g/11 xylanase. biochem biophys res commun 2004;322:277–80.
著作(books)：
1．主编：《基因工程原理与实验指南》，中国轻工业出版社，2010，2．副主编：《固态发酵原理》化学工业出版社，2008，
3．副主编：《酶制剂工艺学》化学工业出版社，普通高等教育“十一五”规划教材，2008
主要荣誉及成果 (honors)：
教学 (education)：
1． 08年度河南农业大学“教书育人”先进个人
2． 09年河南省“教学技能竞赛二等奖”
3． 09年河南农业大学校级精品课程：《基因工程》
4． 09年河南农业大学校级教学成果奖二等奖：“《基因工程》实践教学法研究”
5． 09年河南农业大学第二届大学生实验创新计划“优秀指导教师”
6. 12年河南省优秀硕士论文指导老师
科研 (research)：
1．河南省第十届自然科学优秀学术论文壹等奖：“《the influence of secondary content on thermostability of f/10 xylanase》”，（2010.4）
2．河南省第十届自然科学优秀学术论文贰等奖：“《computational analysis of di-peptides correlated with the optimal temperature in g/11 xylanase》”，（2010.4）
3．河南省第九届自然科学优秀学术论文贰等奖：computational analysis of responsible dipeptides for optimum ph in g/11 xylanase（2006.7）
4．河南省第九届自然科学优秀学术论文贰等奖：principle component analysis in f/10 and g/11xylanase（2006.7）
1． 08年度河南农业大学“教书育人”先进个人
2． 09年河南省“教学技能竞赛二等奖”
3． 09年河南农业大学校级精品课程：《基因工程》
4． 09年河南农业大学校级教学成果奖二等奖：“《基因工程》实践教学法研究”
5． 09年河南农业大学第二届大学生实验创新计划“优秀指导教师”
6. 12年河南省优秀硕士论文指导老师
科研 (research)：
1．河南省第十届自然科学优秀学术论文壹等奖：“《the influence of secondary content on thermostability of f/10 xylanase》”，（2010.4）
2．河南省第十届自然科学优秀学术论文贰等奖：“《computational analysis of di-peptides correlated with the optimal temperature in g/11 xylanase》”，（2010.4）
3．河南省第九届自然科学优秀学术论文贰等奖：computational analysis of responsible dipeptides for optimum ph in g/11 xylanase（2006.7）
4．河南省第九届自然科学优秀学术论文贰等奖：principle component analysis in f/10 and g/11xylanase（2006.7）
